JOURNAL 3248


Journal of Chemical Metrology
VOLUME & ISSUE
Available Online: September 09,2024
PAGES
p.1 - 10
DOI ADDRESS
http://doi.org/10.25135/jcm.112.2406.3248
(DOI number will be activated after the manuscript has been available in an issue.)
STATISTICS
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AUTHORS
  • Kirati Shah
  • Avani Chokshi
  • Niraj Vyas
PDF OF ARTICLE

GRAPHICAL ABSTRACT


ABSTRACT


A simple, effective, and fast reversed-phase high-performance liquid chromatographic method was devised to separate and quantitatively determine quercetin and piperine in herbal extract. the analytes were eluted on the Intersil C18 ODS column (150 mm×4.6 mm, 5μm) as a stationary phase with a gradient elution technique. The mobile phase was composed of a mixture of water (with 0.1% formic acid) and methanol (with 0.1% formic acid) and the gradient program of 30 minutes was optimized. The mobile phase flow rate was adjusted to 1 mL min-1 for 20 μL injection volume. The eluents were detected by the PDA detector at 342 nm wavelength. At the optimized chromatographic conditions, the developed method was validated for the parameters including system suitability, specificity, linearity, accuracy, precision, and robustness per the ICH Q2(R1) guidelines. The developed method was applied for the determination of quercetin and piperine in the prepared herbal extract.

KEYWORDS
  • Herbal extract
  • piperine
  • quercetin
  • RP-HPLC
  • validation

SUPPORTING INFORMATION


Supporting Information
Download File 112-JCM-2406-3248-SI.pdf (150.45 KB)