JOURNAL 3167
Records of Agricultural and Food Chemistry
Available Online: May 09,2024
p.1 - 9
http://doi.org/10.25135/rfac.83.2403.3167 (DOI number will be activated after the manuscript has been available in an issue.)
Viewed 140 times.
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Adel Shehata
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Abdulrahman Alaskar
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Mohammed Alrashed
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Abdulrahman Alzahrani
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Fahad Alkharraa
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Sowailem Alsowailem
GRAPHICAL ABSTRACT
ABSTRACT
Abstract: Sodium benzoate is a widely used food preservatives and its determination receives much interest. This article describes the development and validation of a method using a UV-VIS-NIR Spectrophotometer at 225 nm to determine the mass fraction of sodium benzoate in water for the purpose of characterization of a reference material. The study was carried out in accordance with the ICH and EURACHEM guides using a primary reference material of sodium benzoate of purity 99.98±0.22%. The studied performance characteristics were the limit of detection (LOD), limit of quantification (LOQ), selectivity, linearity, accuracy, precision, recovery and bias. The results obtained were statistically analyzed and the method showed very good linearity in the selected calibration range. The LOD and the LOQ were found 0.19 and 0.57 mg/kg respectively. This method demonstrated very good accuracy ranging from 99.54 to 100.08% and precision, 0.39% RSD, signifying its high reliability in producing precise and accurate results. The validation results also revealed that the method is fit-for-the purpose of determination of sodium benzoate in water.
KEYWORDS- Sodium benzoate
- UV-VIS-NIR Spectrophotometer
- validation
- linearity
- precision
- accuracy
