A New Amide from the Stem Bark of Illiciumdifengpi and Its Anti-inflammatory Activity

A new amide, named ( 2E, 4E)-5-phenyl-N-(2-phenylethyl)-2,4-pentadienamide (1) , together with one known amide, N -2-phenylet hylcinnamide (2) and two known ceramides, 2-​hydroxy-​N-​[(1S,​2R,​3E)​-​2-​hydroxy-​1-​(hydroxymethyl)​-​3-​heptadecenyl]​-pentadecanamide (3), 1-O-(β-D-glucopyranosyl)-(2S,3R,4E,8E)-2-[(2R)-2-hydroxypentadecanoylamino]-4,8-octadecadiene-1,3-diol (4) were isolated from the stem bark of Illicium difengpi. The structures of the isolated compounds were elucidated by analyses of their 1H and 13C NMR, COSY, H S QC, HMBC spectr a and HR-ESI/MS mass spectrometric data. Anti-inflammatory assays with compounds 1‒4 were carried out, compounds 1 and 2 showed significant inhibitory effect on TNF- α release in LPS stimulated RAW 264.7 macrophages.