Anti-Mycobacterium tuberculosis Active Metabolites from an Endophytic Streptomyces sp. YIM65484

In our screening for antitubercular leading compounds, an endophytic Streptomyces sp. YIM65484 was selected by biological assay. Four bioactive metabolites were isolated from this strain. Their structures were determined as (2E,4E)-5-(3-hydroxyphenyl)- penta-2,4- dienamide (1), ergosterol (2), ergosterol peroxide (3) and halolitoralin B (4) by spectral analysis. Compound 1 is a new phenylpentadienamide with its rarely encountered skeleton in natural products. Compounds 1-4 showed selectivity for anti-M. Tuberculosis activity in comparison with other antimicrobial activity. Compound 4 was first isolated from Streptomyces.