Secondary Metabolites and Bioactivity of the Endophytic Fungus Phomopsis theicola from Taiwanese endemic plant

A new cytochalasan named as phomocytochalasin (1), together with five previously identified compounds, cytochalasin H, cytochalasin N, RKS-1778, dankasterone B, cyclo(L-Ile-L-Leu), were isolated from the solid fermentate of Phomopsis theicola BCRC 09F0213, an endophytic fungus isolated from the leaves of an endemic Formosan plant Litsea hypophaea Hayata . The structure of the new compound was established by spectroscopic methods, including UV, IR, HR-ESIMS, and extensive 1D- and 2D-NMR techniques. Among the isolates, cytochalasin N showed NO inhibitory activity with IC 50 values of 77.8 μM . Cytochalasin H showed the progesterone receptor (PR) antagonism with the IC 50 value of 1.42 μM.