Antibacterial and Cytotoxic Activity of Extracts and Isolated Compounds from Myrciariaferruginea (Myrtaceae)

This study evaluated for the first time the antibacterial activity, cell viability and migration ability on   3T3 murine fibroblast  cells of extracts and isolated compounds [lupeol (1), hexamethylcoruleoellagic acid (2) and a mixture of 1 and betulinaldehyde (3)] of Myrciaria ferruginea. In antibacterial assays extracts were susceptible only against S. aureus (MIC 500 μg/mL) and S. epidermidis (MIC ranging from 7.8 to 500 μg/mL) and compounds 1-3have shown no significant activity. In trials for c ell viability, with exception of MeOH-H 2O fraction from leaves (viable cells > 90%), both the crude extract and other fractions showed inhibition of cell growth (viable cells ≤ 80% at 15.625 and 31.25 μg/mL); while the samples from stems, with the exception of CHCl 3 fraction that showed strong cytotoxic effect at the lowest concentration tested (15.625 μg/mL), the other fractions were not cytotoxic. Compounds (1-3) inhibited cell viability in dose dependent manner (15.625 to 500 μg/mL). Mixture containing 1 and 3 showed inhibitions only in concentrations greater than 62.5 μg/mL while compound 2 decreased from the lowest concentration tested. In scratch wound assay, these compoundsnot increased the population of fibroblasts at concentrations less than 62.5 μg/mL.