JOURNAL 1132
Records of Natural Products
Year: 2019 Issue: 6 November-December
p.475 - 482
Viewed 2435 times.
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Zhenggang Yue
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Rui Zhou
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Yihan He
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Hongbo Xu
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Yalei Pan
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Liyan Lei
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Pei Xie
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Zhishu Tang
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Jinao Duan
GRAPHICAL ABSTRACT
ABSTRACT
Pseudolarix amabilis (Nelson) Rehd, is a monotypic genus plant belonging to the family Pinaceae. The root bark , known as “Tu-Jin-Pi” has been used for the treatment of skin diseases. During our activity screening, the water-soluble part of the 70% EtOH extract of P. amabilis bark showed excellent inhibitory bioactivities on PTP1B enzyme, leading to the phytochemical isolation of the root bark of P. amabilis. Three oleanane-type compounds (1-3) and seven phenolic compounds (4-10) were isolated, in which oleanolic acid 3-O-β-D-glucuronyl-6′-ethyl ester (1) was identified as a new saponin. The chemical structures of these compounds were elucidated by 1D/2D nuclear magnetic resonance and high resolution mass spectra. In addition, their pharmacological inhibitory bioactivities on PTP1B enzyme were evaluated, and the three oleanane-type compounds 1-3 exhibited inhibitory bioactivities with IC50 values of 1.90 ± 0.37, 19.15 ± 0.10 and 10.44 ± 0.59 μM, respectively.
KEYWORDS- Pseudolarix amabilis
- PTP1B inhibitors
- Isolation and identification
- Oleanane-type compounds
- Phenolic compounds
- Oleanolic acid 3-O-β-D-glucuronyl-6′-ethyl ester
