JOURNAL 1477
Records of Natural Products
Year: 2020 Issue: 5 September-October
p.326 - 339
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Otilija Keta
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Milena Deljanin
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Vladana Petkovic
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Gordana Zdunić
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Teodora Janković
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Jelena Živković
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Aleksandra Ristic Fira
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Ivan Petrović
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Katarina Šavikin
GRAPHICAL ABSTRACT
ABSTRACT
The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.
KEYWORDS- Punica granatum L.
- peel extract
- phenolic compounds
- cytotoxicity
- cancer cells
