JOURNAL 3319
Records of Natural Products
Year: 2024 Issue: 6 November-December
p.629 - 642
Viewed 571 times.
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Luis D. Herrera-Sanabria
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Ana K. Villagómez-Guzmán
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José Herrera-Camacho
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Edgar A. Estrella-Parra
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José G. Avila-Acevedo
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Rosa E. del Río
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Gabriela Rodríguez-García
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Tzasna Hernández-Delgado
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Mario A. Gómez-Hurtado
GRAPHICAL ABSTRACT
ABSTRACT
The Trixis genus (Asteraceae) is involved in traditional medicine from Latinoamerica. In Mexico, Trixis michuacana Lex. var. michoacana and T. michuacana var. longifolia are used in the P'urhépecha ethnomedicine. The analysis of their volatile compounds by gas chromatography/mass spectrometry (GC-MS) is described herein. The sesquiterpene compounds were the main constituents. β-Caryophyllene (10.80%), germacrene D (7.93%), β-elemene (7.42%), and γ-elemene (7.37%) highlighted from var. michuacana, while β-elemene (10.02%), α-copaene (9.91%), β-caryophyllene (8.97%), germacrene D (7.40%), and γ-elemene (6.89%) were the major constituents from var. longifolia. A systematic analysis of the chemical components provided an approach to the biosynthetic pathway for volatile metabolite production. Antimicrobial assays provided scientific support for the traditional use of the plants. In-vitro assays using Escherichia coli (ATCC 25922), E. coli (82MR), Klebsiella pneumoniae (ATCC 13883), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 29213), S. aureus (23MR), S. epidermidis (ATCC 12228), S. epidermidis, Candida albicans (17MR), C. glabrata, and C. tropicalis were achieved. These assays revealed MIC values >2 mg/mL when observed bacterial inhibition for both Trixis species, except for var. michuacana against S. epidermidis ATCC 12228, which was 0.5 mg/mL. These results could partially justify the use of the studied plants in ethnomedicine.
KEYWORDS- Trixis michuacana
- Asteraceae
- antimicrobial activity
- biosynthetic approach
