JOURNAL 2985
Journal of Chemical Metrology
VOLUME & ISSUE
Year: 2023 Issue: 2 July-December
Year: 2023 Issue: 2 July-December
PAGES
p.249 - 256
p.249 - 256
STATISTICS
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Viewed 829 times.
GRAPHICAL ABSTRACT
ABSTRACT
Amount of α-escin and β-escin in cosmetic cream formulations were determined by simple reverse phase HPLC method by using the C18 column at 228 nm. The mobile phase is composed of acetonitrile-water (60:40) at pH 2.4. The method validation study was carried out based on the ICH recommendations and linear regression equations were determined as y=1548.3x+340.7 and y=1521.3x-91.9, for the analytes, respectively. While LOD values of the method were determined for α-escin and β-escin as 0.003 µg/mL and 0.027 µg/mL, LOQ values were determined as 0.01 µg/mL and 0.08 0.01 µg/mL, respectively
KEYWORDS- Horse chesnut
- α-escin
- β-escin
- HPLC-UV
- Validation