JOURNAL 198


Records of Natural Products
VOLUME & ISSUE
Year: 2017 Issue: 1 January-February
PAGES
p.9 - 16
STATISTICS
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AUTHORS
    Alessandra Piras, Hanen Marzouki, Danilo Falconieri, Silvia Porcedda, Maria José Gonçalves, Carlos Cavaleiro and Ligia Salgueiro
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GRAPHICAL ABSTRACT


ABSTRACT


Volatile oils composition from leaves and ripe fruits of pink pepper (Schinus terebinthifolius Raddi) growing in Tunisia were investigated using GC-FID and GC-MS techniques. Volatile oil extraction was achieved by hydrodistillation (HD) using a Clevenger apparatus and by supercritical fluid extraction (SFE) using carbon dioxide. All plant organs, gave SFE extracts chiefly composed by a -pinene, a -phellandrene, b -phellandrene, germacrene D and bicyclogermacrene. In the case of the fruits, both extraction techniques gave volatile oils of similar composition; whereas the comparison between the HD and SFE leave oils revealed important differences in the content of a -pinene (6.1 % vs traces), a -phellandrene (22.7 % vs 0.8 %) and b -phellandrene (14.6 % vs 1.2 %). All volatile samples were evaluated against yeasts and dermatophyte strains, being more active against Cryptococcus neoformans, particularly the volatile oil from the fruits, with MIC values of (0.32-0.64) mg/mL.Moreover, this oil revealed an inhibitory effect on germ tube formation in C. albicans at sub-inhibitory concentration. At the concentration of MIC/8 the inhibition of filamentation was more than 70 %.

KEYWORDS
  • Schinus terebinthifolius
  • supercritical carbon dioxide extracts
  • essential oil
  • antifungal activity