HPLC Fingerprinting of Sennosides in Laxative Drugs with Isolation of Standard Substances from Some Senna Leaves

Senna leaves are one of the oldest medicinal herbs and they are used as laxative. Herbal teas which contain senna leaves are most commonly used to promote weight loss. The quality control of slimming teas which contain Senna leaves and also pharmaceutical preparations including Senna extract enriched by sennoside B was achieved by HPLC fingerprinting method. While the presence of sennoside A and B in laxative drugs was proved, it was seen to be devoid of sennosides in slimming teas. Kaempferol 3-O-β-D-gentiobioside (1), aloe-emodine 8-O-β-D-glucopyranoside (2), rhein 8-O-β-D-glucopyranoside (3), torachrysone 8-O-β-D-glucopyranoside (4), isorhamnetine 3-O-β-D-gentiobioside (5) were also isolated from Senna leaves.