JOURNAL 2935
Records of Natural Products
Year: 2024 Issue: 1 January-February
p.165 - 170
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GRAPHICAL ABSTRACT
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ABSTRACT
Nineteen compounds were purified from leaves of Ficus carica Linn. using various separation techniques such as recrystallization, column chromatography filled with silica gel, Sephadex LH-20, and MCI, and semi-preparative HPLC. By analysis of NMR, MS, and data comparison with those reported from literatures, their structures were identified as umbelliferone (1), psoralen (2), furopinnarin (3), 6,7-furano-hydrocoumaric acid (4), (E)-3-[5-(6-hydroxy) benzofuranyl] propenoic acid (5), (E)-3-(6-hydroxy-4-methoxy-5-benzofuranyl) propenoic acid (6), nodakenetin (7), oxypeucedanin hydrate (8), dihydrofurocoumarin (9), (E)-4-hydroxy-3,3,5-trimethy1-4-(3-oxobu-1-en-1-yl)-cyclohexan-1-one (10), dehydrovomifoliol (11), 4,5-dihydroblumenol A (12), blumenol A (13), 5-hydroxy-4′,7-dimethoxyisoflavone (14), cajanin (15), loliolide (16), indole-3-carboxaldehyde (17), 1H-indole-3-carboxylic acid (18), vitamin E quinone (19). Their α-glucosidase inhibitory activities were evaluated by IC50 values. Compounds 15 and 19 showed obvious α-glucosidase inhibitory activity with IC50 of 71.1±1.56 µM and 49.3 µM±1.21, respectively, and by the kinetics of enzymatic reaction, the inhibition type by which compound 15 acting against α-glucosidase was inferred as anticompetitive. Moreover, preliminary structure-activity relationship was recapticulated from the tested compounds.
KEYWORDS- Ficus carica Linn.
- coumarin
- α-glucosidase inhibitor
- enzyme kinetics