JOURNAL 3343
Records of Natural Products
Available Online: November 24,2024
p.1 - 11
http://doi.org/10.25135/rnp.487.2410.3343 (DOI number will be activated after the manuscript has been available in an issue.)
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- Thanh Q. C. Nguyen
- Ta Thanh Hong
- Vo Thanh Khang
- Ho Thi Nhu Y
- Duy Toan Pham
- Dang Huynh Giao
- Truong Thi Tu Tran
- Kaeko Kamei
GRAPHICAL ABSTRACT
ABSTRACT
Inflammation is one of the basic pathological processes due to the adaption of immune system against infection or irritation. Since non-steroidal anti-inflammatory drugs (NSAIDs) cause many side effects, investigation of anti-inflammatory natural products promising to provide novel therapeutic agents. Here, Launaea sarmentosa (Willd.) Kuntze, a medicinal herb applied to treat inflammation diseases, was investigated for its anti-inflammatory components to find new therapies for inflammatory syndromes. This study indicated that ethyl acetate fractional extract reduced the expression of pro-inflammatory cytokines, representing the highest activity for both NO radical-scavenging and NO secretion inhibition during LPS-stimulated RAW264.7 macrophages. Besides, five anti-inflammatory compounds, including succinic acid, quercetin, 2(4-hydroxyphenyl)acetic acid, luteolin-7-O-β-D-glucopyranoside, and quercetin-3-O-rutinoside, were isolated and elucidated the structure according to 1D and 2D- NMR. Among these compounds, succinic acid and 2(4-hydroxyphenyl)acetic acid were first reported in this species. Moreover, this study indicated that the presence of these compounds, typically quercetin and luteolin-7-O-β-D-glucopyranoside, enhanced anti-inflammatory ability via deactivation of NF-кB/MAPK pathway to mitigate the expression of IL-6. Hence, this study contributed the initial evidence of anti-inflammatory constituents from Launaea sarmentose and highlighted an approach to discovering natural items or phytotherapeutic agents.
KEYWORDS- Anti-inflammatory
- Launaea sarmentosa
- mitogen-activated protein kinase
- nuclear factor kappa B