Records of Natural Products

Articles In Press

Short Report

1) A New Acyclic Compound from the Medicinal Plant Tinospora sinensis

Rec. Nat. Prod. (2025) in press ; 1 - 5
by Mengying Zhang and Lijing Cai

In this study, chemical investigation of the aerial parts of the medicinal plant Tinospora sinensis yielded five compounds (1-5), including a new acyclic compound (1) and four known compounds (2-5). Their structures were determined by extensive analysis of the spectroscopic data, including 1D (1H and 13C NMR) and 2D NMR data (HSQC, COSY, and HMBC). Compounds 3-5 were identified to be stearic acid (3), abscisic acid (4), and blumenol A (5) based on comparisons of the NMR data with those reported in the literature. The NMR data for 2-hydroxy methyl stearate (2) were reported for the first time in CDCl3 in this study. The compounds were evaluated for their antitumor potential against A549 cell line, while all were inactive (25 mM). Besides, compound 1 demonstrated marginal inhibition (19.35%) of LPS-induced NO production in RAW 264.7 cells (50 mM).

DOI
http://doi.org/10.25135/rnp.530.2505.3536
Keywords
Tinospora sinensis acyclic compound NMR
Available online: July 26, 2025
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Short Report

2) Phytochemical Characterization, Antifungal Activity and Docking Investigation of The Isolated Compounds from Lippia callicarpifolia

Rec. Nat. Prod. (2025) in press ; 1 - 6
by Ana K. Villagómez-Guzmán , Ana M. García-Bores , Israel Valencia , Miriam Arlette López-Pérez , Edgar Antonio Estrella-Parra , José G. Avila-Acevedo and Tzasna Hernández-Delgado

Bioassay-guided fractionation of extracts of aerial parts from Lippia callicarpifolia usingvia chromatographic techniques led to the isolation of two D:C-friedours-7-ene compounds, namely D:C-3-oxo-friedours-7-en-28-oic acid (1) and a new callicarpifolic acid (2). Furthermore, additional known compounds, β-sitosterol glucoside (3) and genkwanin (4) were also identified. The structures of the natural products were established by 1D and 2D NMR, IR, and spectrometric analysisanalyses. In the antifungal assays, Fusarium sporotrichioides NRLL was sensitive to compounds 1 and 4 in the concentration range of 0.250-–1 mg/mL. The docking investigation revealed high inhibition of the trichodiene synthase enzyme by compound 4.

DOI
http://doi.org/10.25135/rnp.535.2502.3432
Keywords
Lippia callicarpifolia Fusarium sporotrichioides genkwanin callicarpifolic acid
Available online: July 26, 2025
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Original Article

3) Isolation and Identification of Diterpenoids from Euphorbia helioscopia and Their Anti-inflammatory Activities

Rec. Nat. Prod. (2025) in press ; 1 - 8
by Liping Dai , Jinke Zheng , Jun Chi , Bin Cao and Huili Li

Phytochemical investigation of 70% ethanol extract of Euphorbia helioscopia led to eight diterpenoids, including four jatrophane-type (14), three abietane-type (57) and one lathyrane-type (8). Among them, (5E,11E)-3β-benzoyloxy-7β,14α,15β-trihydroxyjatropha-9-one (1) was a new jatrophane diterpenoid. An extensive array of spectral analyses, including HR-ESI-MS, IR, UV, 1D and 2D NMR, as well as quantum chemical calculations of ECD curve, were utilized for their structural elucidation. Bioassay results showed that compounds 23 and 68 could significantly inhibit NO release on LPS-induced RAW 264.7 cells, especially for jatrophane diterpenoid 3 and abietane diterpenoid 8 that showed stronger activities with the IC50 values of 9.16 ± 0.70 and 5.74 ± 0.27 μM.

DOI
http://doi.org/10.25135/rnp.533.2502.3446
Keywords
Euphorbia helioscopia diterpenoids anti-inflammatory
Available online: July 26, 2025
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Short Report

4) A New Lignan with Potential Anti-inflammatory Activity from Notopterygium incisum

Rec. Nat. Prod. (2025) in press ; 1 - 6
by Li-lian Zhao , Su Hu , Xin-Yu Li , Yun Deng , Li-jun Huang and Dale Guo

NNotopterygium incisum is widely used in clinical practice because of its complex chemical composition and remarkable pharmacological effects. In this study, a previously undescribed lignan (1) was isolated from N. incisum. Its structure was evaluated through examinations of the NMR, HREIMS data and ECD calculation. Compound 1 demonstrated significant anti-inflammatory potential by inhibiting the secretion of nitric oxide (NO) in lipopolysaccharide (LPS)-induced macrophages, with an IC50 value of 3.57 μM.

DOI
http://doi.org/10.25135/rnp.2505.3518
Keywords
Notopterygium incisum lignan anti-inflammatory activity
Available online: July 14, 2025
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Short Report

5) Eupbenzofuranside C: A New Benzofuranside with Dual Inhibitory Activities against α-Glucosidase and PTP1B from Eupatorium chinense L.

Rec. Nat. Prod. (2025) in press ; 1 - 6
by Ting Yan , Ye Deng , Yanjie Zeng , Ruixue Jing and Han Shen

A new benzofuranside compound (eupbenzofuranside C) was isolated from the n-butanol fraction of ethanol extract of Eupatorium chinense L. along with three known compounds. The structures of the isolated compounds were elucidated by 1D and 2D NMR techniques, mass spectrometry and circular dichromism (CD). The isolated compounds were investigated for their α-glucosidase and PTP1B inhibitory activities by p-nitrophenyl-β-galactopyranoside method and p-nitrophenyl phosphate method, respectively. Although eupbenzofuranside C showed potential dual inhibitory activity against α-glucosidase and PTP1B with IC50 values of 40.07±0.38 μg/mL and 39.37±0.36 μg/mL, respectively, these values were determined to be far from the data of the positive control acarbose. In contrast, compound 2 showed inhibitory activities against both α-glucosidase and PTP1B with IC50 (μg/mL) values of 4.83±0.29 and 17.29±0.17, which were closer to acarbose. Compounds 3 and 4 showed no inhibition in both tests (IC50 > 50 μg/mL). In addition, the interactions of compound 1 with α-glucosidase and PTP1B were analyzed using the active site analysis for computer-aided drug design.

DOI
http://doi.org/10.25135/rnp.527.2503.3460
Keywords
Eupatorium chinense L bioactive components inhibitory activity α-glucosidase PTP1B
Available online: July 11, 2025
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Short Report

6) In Vitro and In Silico Evaluation of Compounds from Washingtonia filifera as Acetylcholinesterase Inhibitors

Rec. Nat. Prod. (2025) in press ; 1 - 6
by Aalaa Salem , Fatma Abdel Bar , El-sayed M. Marwan , Amal F. Soliman , Saleh H. El-sharkawy and Amira Mira

Nine known compounds were isolated from the fruitless bunches of Washingtonia filifera, an underutilized agricultural waste, using NMR and mass spectrometry. The identified compounds included β-sitosteryl oleate (1), oleic acid (2), β-sitosterol (3), threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol (4), syringaresinol (5), diosmetin (6), tricin (7), daucosterol (8), and luteolin-7-O-β-D-glucoside (cynaroside) (9). In vitro acetylcholinesterase (AChE) inhibition assays revealed that compound 5 exhibited the strongest activity (IC50 = 29.75 µM), followed by compounds 4 and 6. Docking studies indicated significant interactions of the active compounds with key AChE residues, particularly Trp86 and Tyr341. ADME predictions further supported the drug-likeness of compounds 4 and 5. These results highlight the significance of W. filifera agricultural waste as a source of bioactive compounds, particularly with neuroprotective effects.

DOI
http://doi.org/10.25135/rnp.531.2504.3484
Keywords
Washingtonia filifera California fan plam fruitless bunch acetylcholinesterase phenolics lignan
Available online: July 11, 2025
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Short Report

7) Fusasolpolyol A, An Unreported Polyhydroxy Compound Isolated from the Sargassum thunbergii-Derived Endophytic Fungus Fusarium solani 2024f-xx

Rec. Nat. Prod. (2025) in press ; 1 - 6
by Lian-Cheng Xu , Wei-Huan Luo , Shan Liu , Jian-xin Ye and Yu Chen

Fusarium solani 2024f-xx, an endophytic fungus derived from the marine brown algae Sargassum thunbergii, was chemically studied. As a result, two polyhydroxy compounds, namely fusasolpolyol A (1) and (4E,8E,12E)-2,3,7,11-tetrahydroxy-2,4,6,8,10,12-hexamethyltetradeca-4,8,12-trienoic acid (2), as well as two known drimane-type sesquiterpenoids (3 and 4) were isolated and identified. The structures of the isolated compounds were ascertained by means of specific spectroscopic methods (mainly determined by HRESIMS and 1D/2D NMR data). Compound 1 was identified as a new compound. In the cytotoxic assays, compound 2 revealed moderate activity against the human gastric carcinoma cell MKN-45 and the human pancreatic cancer cell PATU8988T, with the respective IC50 values of 19.6 ± 1.2 μM and 26.3 ± 0.9 μM.

DOI
http://doi.org/10.25135/rnp.520.2504.3506
Keywords
Fusarium solani endophytic fungus secondary metabolites polyhydroxy compound cytotoxic activity
Available online: May 29, 2025
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Review Article

8) Chemical Constituents and Pharmacology of Fomes fomentarius: A Systematic Review

Rec. Nat. Prod. (2025) in press ; 1 - 30
by Chuanming Gong , Miaomiao Xu , Haonan Xu , Yuze Li , Chong Deng , Yi Jiang , Xiaomei Song and Dongdong Zhang

This paper mainly provides a systematic review of the chemical constituents and biological activities of Fomes fomentarius. As a widely distributed medicinal fungus, F. fomentarius has a long history of application in traditional medicine. Rich in chemical constituents, it contains over a hundred compounds such as steroids, triterpenes, and fatty acids. Pharmacological studies have demonstrated that this fungus exhibits multiple activities, including antitumor, immunomodulatory, antioxidant, antibacterial, anti-inflammatory and analgesic, antidiabetic, anti-hypoxic, and hepatoprotective effects. However, our current understanding of its action mechanisms and the synergistic effect among components remains limited. This review provides crucial references for further research and development of F. fomentarius.

DOI
http://doi.org/10.25135/rnp.521.2503.3466
Keywords
Fomes fomentarius chemical constituents pharmacological activities Pyropolyporus fomentarius
Available online: May 29, 2025
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Short Report

9) Trichothecene Sesquiterpenes with Anti-osteosarcoma Cytotoxicity from the Fungus Fusarium sp. XPW68

Rec. Nat. Prod. (2025) in press ; 11 - 5
by Huihuang Peng , Rui Chen , Yanxia Zhang , Linsa Zhou and Jie Lin

Fusarinene A (1), a previously undescribed trichothecene sesquiterpene, and three known compounds, 4β-hydroxytrichotheca-9,12-diene (2), trichodermol (3), and trichodermin (4), were isolated from the extract of Fusarium sp. XPW68. Their structures and relative configurations were identified by detailed analysis of nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectrometry (HRESIMS) data. In the cytotoxic assay, compounds 1-4 exhibited selective or potent inhibition against five human osteosarcoma cell lines including 143B, MG-63, SaOS-2, SW1353, and U2OS, with IC50 values ranging from 0.8 to 48.2 μM.

 

 

DOI
http://doi.org/10.25135/rnp.519.25.04.3479
Keywords
Fusarium trichothecene sesquiterpene structure elucidation cytotoxic activity
Available online: May 22, 2025
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Original Article

10) Beyond the Traditional Applications of Raspberry (Rubus idaeus) Leaf: An in vitro, in vivo and in silico Study

Rec. Nat. Prod. (2025) in press ; 1 - 15
by Marija Vukelic-Nikolic , Anđela Dragićević , Nikola Stojanović , Perica Vasiljević and Dragana Pavlović

The consumption of raspberry (Rubus idaeus L.) leaves has a long tradition, especially as a "general pregnancy tea", although the scientific data are insufficient and contradictory. Phytochemical comparation of extracts from cultivated and wild raspberry leaves, in silico prediction of their biological activities and acute toxicity followed by in vitro antiradical activity and effects on the viability/proliferation of HeLa cells and isolated rat uterus were performed. Leaves from cultured (v. Polka) and wild individuals were extracted with distilled water, 70% v/v ethanol or 70% v/v methanol. All samples exhibited high polyphenol content and antiradical activity, with the 70% v/v ethanol extract of wild R. idaeus showing the strongest free radical scavenging ability. In silico analyzes predicted that raspberry leaves possess numerous compounds with anti-inflammatory, apoptosis-agonistic, antinociceptive and NO signaling-related activities. Potentially toxic levels of the tested compounds could not be achieved with regular tea drinking. The tested extracts have no noticeable effects on the viability/proliferation of HeLa cells. The effects on spontaneous contraction of the isolated rat uterus were modest. Although safety is not a concern, further studies are needed to justify or deny the efficacy of raspberry leaf tea in folk medicine for healthy pregnancy and easy delivery.

DOI
http://doi.org/10.25135/rnp.493.2410.3352
Keywords
R. idaeus L. leaves extracts phytochemical comparation in silico prediction HeLa cell viability/proliferation uterus contraction
Available online: January 22, 2025
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